Fig. 7
From: Osa-miR7695 enhances transcriptional priming in defense responses against the rice blast fungus
Expression of PR and lignin biosynthesis genes in WT-Az and MIR7695-Ac plants during blast infection. Plants were sprayed with a M. oryzae spore suspension. Leaves were collected at the indicated times (24, 48, 72 hpi). a-b Expression analysis of (a) PR (OsPR1b, OsPBZ, OsPR10b) and (b) lignin (OsCAD2, OsCOMT1) genes determined by RT-qPCR. Data are mean ± SE (n = 3; each sample consisted of a pool of 3 individual leaves). Mock-inoculated (control, grey) and M. oryzae-infected (red) plants. Time point used for RNAseq analysis (48 h) is labeled with a thick line in the x-axis. c Heatmap showing log2 FC for each transcript and each time (infected vs. control) as determined from RT-qPCR values (a-b). Upregulated (purple) and downregulated (green). Dots indicate significant differences (infected vs control) (Student t test, p < 0.05)