Fig. 3
From: Osa-miR7695 enhances transcriptional priming in defense responses against the rice blast fungus
Histochemical detection of iron (Fe) in wild-type (O. sativa cv. Tainung 67, japonica) and MIR7695-Ac mutant plants during M. oryzae infection. Wild-type and MIR7695-Ac mutant plants at the three-leaf stage were (a) mock-inoculated or (b) inoculated with M. oryzae spores. At 24, 48, 72 h post-inoculation (hpi), the third leaf of each plant was stained with Perls. Iron is detected as blue precipitates. Representative images of one experiment are shown (n = 4). Three independent infection assays were performed with similar results. Scale bar: 100 μm. st, stomata