Development and characterization of an EMS-mutagenized wheat population and identification of salt-tolerant wheat lines

Table 2 The number of transitions and transversions observed for the mutagenized lines using SAMtools and Freebayes

Type of substitution	Base to base conversion	OA70		OA42
Type of substitution	Base to base conversion	SAMtools	FreeBayes	SAMtools	FreeBayes
Transition	G → A	219,110	220,536	250,786	228,122
	C → T	227,802	227,030	221,062	206,493
	T → C	46,420	81,548	33,158	64,847
	A → G	46,417	83,720	33,168	62,467
Transversion	C → A	12,932	19,003	9454	14,581
	G → T	12,991	19,037	9022	14,200
	A → C	10,707	18,810	7492	14,205
	T → G	10,612	19,006	7566	14,385
	G → C	10,160	16,774	7116	12,942
	C → G	10,053	16,967	6967	12,789
	T → A	7681	12,201	5916	9873
	A → T	7570	12,272	5994	9936

ISSN: 1471-2229