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Table 1 Summary of ROI from the PacBio Sequel platform

From: Single-molecule real-time sequencing facilitates the analysis of transcripts and splice isoforms of anthers in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

Sample

Library

Cell

CCS

5′ primer

3′ primer

Poly-A

Full-length

FLNC

Mean FLNC length (bp)

Ant 1-2 k

1-2 K

D01

418,042

390,507

386,739

365,528.00

352,741

329,694

1499

Ant 2-3 K

2-3 K

E01

355,765

296,959

290,222

270,230.00

246,734

243,747

2324

Ant > 3 K

3 K+

A01

324,312

298,840

295,242

279,866.00

263,806

253,881

3288

Total

–

–

1,098,119

986,306

972,203

915,624.00

863,281

827,322

–