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Table 1 Summary of ROI from the PacBio Sequel platform

From: Single-molecule real-time sequencing facilitates the analysis of transcripts and splice isoforms of anthers in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

SampleLibraryCellCCS5′ primer3′ primerPoly-AFull-lengthFLNCMean FLNC length (bp)
Ant 1-2 k1-2 KD01418,042390,507386,739365,528.00352,741329,6941499
Ant 2-3 K2-3 KE01355,765296,959290,222270,230.00246,734243,7472324
Ant > 3 K3 K+A01324,312298,840295,242279,866.00263,806253,8813288
Total1,098,119986,306972,203915,624.00863,281827,322