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Fig. 2 | BMC Plant Biology

Fig. 2

From: Single-molecule real-time sequencing facilitates the analysis of transcripts and splice isoforms of anthers in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

Fig. 2

Bioinformatics analysis. a Flow chart of PacBio Sequel platform. b Illustration of FLNC mapping and PID calculation. m: match. M: mismatch; S: soft-clipping. H: hard-clipping. D: deletion; I: insertion. The calculation formulas of PID were as follow: local PID = m/ (m + M + D + I), gobal PID = m/ (m + M + S + H + D + I). c Classification map of AS events. (M)SKIP: (cassette exons) exon skipping; (M)IR: retention of (multiple) single introns; AE: alternative exon ends (5′, 3′ or both); X(M)SKIP: approximate (cassette exons) exon skipping; X(M)IR: approximate retention of (multiple) single introns; XAE: approximate alternative exon ends; d Schematic diagram of fusion transcripts detection

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BMC Plant Biology

ISSN: 1471-2229

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