Fig. 7

GmWRKY40 interacts with JAZ proteins. a Diagram of the GmWRKY40 protein functional domains and schematic of the amino acid mutation of WRKYGQK domain or Zinc finger domain. mWRKY indicate WRKY domain mutation; mZinc-finger indicate Zinc-finger domain mutation. b Yeast-two-hybrid assays. The Gal4 DNA binding domain was fused with GmWRKY40 (shown as BD-WRKY40, BD-mWRKY or BD-mZinc-finger) and the Gal4 activation domain was fused with JAZ1, JAZ2 or JAZ3 (shown as AD-JAZ1, AD-JAZ2 or AD-JAZ3). The Gal4 DNA binding domain expressed by pGBKT7 was used as a negative control. 0.8, 0.08, and 0.008 are the bacteria diluted concentration gradient