Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben

Table 1 Distribution of cells according to cell length (μm) 48 h after treatment with cellulose biosynthesis inhibitors, inhibitors of calcium transport and auxins

Treatment	Percentage of cells according to cell length^a
Treatment	10–40 μm	40–60 μm	> 60 μm
Control (Methanol)	44.0 ± 11.7	33.4 ± 2.4	26.5 ± 9.3
Thaxtomin A	75.5 ± 2.2*	22.6 ± 0.2	4.1 ± 0.2*
Isoxaben	67.9 ± 4.7*	27.1 ± 1.5	10.9 ± 6.2*
LaCl₃	92.6 ± 4.3*	4.7 ± 1.8*	2.7 ± 0.6*
RR	85.8 ± 5.6*	12.8 ± 2.7*	1.5 ± 1.0*
2,4-D	89.6 ± 6.3*	7.2 ± 3.3*	3.2 ± 2.8*
IAA	72.1 ± 8.6*	17.5 ± 5.7*	10.4 ± 4.6*

^aCells stained with trypan blue were visualized by light microscopy and photographed. Cell dimensions were measured using Fiji software [37] for 300 cells per condition. Results show the mean percentage of cells in each category of length from 3 replicates ± SD. (*) indicates statistically significant difference with control in each category (t-test followed by Holm-Šídák method, p < 0.05)

ISSN: 1471-2229