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Fig. 3 | BMC Plant Biology

Fig. 3

From: Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben

Fig. 3

Inhibition of auxin transport (efflux) protects from CBI-induced cell death. Percentage of dead cells in Arabidopsis cell cultures 72 h after addition of auxin transport inhibitor and/or CBI. a Cell death was evaluated after treatment with thaxtomin A (TA; 1 μM) and in cells pretreated for 30 min with 10 μM of different auxin transport inhibitors: 2,3,5-triiodobenzoic acid (TIBA) and 1-N-naphthylphthalamic acid (NPA) as efflux inhibitors, 2-naphthoxyacetic acid (NOA) and 3-chloro-4-hydroxyphenylacetic acid (CHPAA) as influx inhibitors prior to TA treatment. b Cell death was evaluated after treatment with isoxaben (IXB; 1 μM) and in cells pretreated for 30 min with the different auxin transport inhibitors prior to IXB addition. Ctrl = control cells treated with equal volume of methanol as treated samples. Each value is the mean of n = 15 (100 cells each) ± SD. Statistically different values (t-test followed by Holm-Šídák method, p < 0.05) are indicated by different letters

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BMC Plant Biology

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