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Fig. 1 | BMC Plant Biology

Fig. 1

From: Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben

Fig. 1

Calcium influx is required for the CBI-induction of cell death. Percentage of dead cells in Arabidopsis suspension cells 48 h after the addition of calcium transport inhibitors. a and c Cells were treated with thaxtomin A (TA; 1 μM), isoxaben (IXB; 1 μM) or lanthanum chloride (LaCl3; 500 μM) alone, or pretreated with LaCl3 (500 μM) 30 min before adding TA or IXB. b and d Cells were treated with TA (1 μM), IXB (1 μM) or ruthenium red (RR; 50 nM) alone, or pretreated with 50 nM of RR 30 min before adding TA or IXB. Ctrl = control cells treated with equal volume of methanol as treated samples. Each value is the mean of n = 15 (100 cells each) ± SD. Statistically different values (t-test followed by Holm-Šídák method, p < 0.05) are indicated by different letters

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