Fig. 8
Analysis of ROS accumulation by CM-H2DCFDA and NBT staining. ROS accumulation was analyzed in wild-type and 35S::FSD3S plants by CM-H2DCFDA (a) and NBT staining (b). CM-H2DCFDA staining was performed in the leaves and roots of wild-type and 35S::FSD3S plants grown on 1/2 MS media for 2 weeks. For NBT staining, 6-week-old rosette leaves collected from the indicated plants were used. c Interaction between FSD3S and pTAC10. A Co-IP result showing that both FSD3 and FSD3S interact with pTAC10. Protoplasts isolated 35S::FSD3-GFP and 35S::FSD3S-GFP plants were transformed with 35S::pTAC10-HA plasmid. HA antibody was used to pull down immune complex and GFP antibody was used to detect interaction of pTAC10-FSD3 or pTAC10-FSD3S. Scale bars = 100 μm in (a) and 2 mm in (b)