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Fig. 2 | BMC Plant Biology

Fig. 2

From: An isoform of the plastid RNA polymerase-associated protein FSD3 negatively regulates chloroplast development

Fig. 2

SOD activity of FSD3S proteins. To analyze SOD activity of FSD3S and FSD3, an in-gel SOD activity assay was performed using MBP-FSD3S and MBP-FSD3. MBP-fused recombinant FSD3S and FSD3 expressed in E. coli were purified with amylose resin. Samples containing sixteen, four, and one microgram of FSD3S or FSD3 protein were loaded on 8% native-PAGE for the test of SOD activity. Intensity indicates the relative SOD activities of FSD3 and FSD3S, which were quantified using Image J software. Coomassie brilliant blue (CBB) staining was used for loading controls

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