Fig. 8

Phenotype and RT-PCR analysis of transgenic Arabidopsis plants. a Comparison of rosette leaves (RL) in wt and transgenic lines shows that 35S:PhLSH7b-2 contains more rosette leaves than others. The letters indicate the result of statistical differences. b Comparison of flowering time (FT) in wt and transgenic lines shows that 35S:PhLSH7b-2 is later flowering than others. The letters indicate the result of statistical differences. c, d qPCR analysis shows the expression level of transgenes in wt and transgenic Arabidopsis lines. 35S:PhLSH7a-11 has higher expression level than other 35S:PhLSH7a lines (c); 35S:PhLSH7b-2 has higher expression level than other 35S:PhLSH7b lines (d). The phenotype changes were largely related to the expression level of transgenes (Fig. 7). e, f Semi quantitative RT-PCR analysis shows the expression level of transgenes in wt and transgenic Arabidopsis lines consistent with the results of qPCR. There is no any interference with the two endogenous genes, AtLSH7/8 (AtLSH8 cannot be detected, so its semi-quantitative results are not displayed)