Fig. 2

Amino acid sequence alignment of GGP proteins from a range of graminaceous species. Green, olive green, yellow and white background colour represents 100%, 80 to 100%, 60 to 80%, and less than 60% conservation of amino acids between species, respectively. The HIT motif (HφHφQ, where φ is a hydrophobic amino acid) of the HIT protein superfamily and the KKRP NLS are outlined in red. The 13 residues across the protein that distinguish GGP1 proteins from GGP2 proteins within the graminaceous species are indicated by an asterisk. The prefixes for the graminaceous species are as follows: Aet is Aegilops tauschii; Bd is Brachypodium distachyon; Hv is Hordeum vulgare; Os is Oryza sativa; Sb is Sorghum bicolor; Ta is Triticum aestivum; and Zm is Zea mays