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Fig. 6 | BMC Plant Biology

Fig. 6

From: Genome-wide identification of the Dicer-like family in cotton and analysis of the DCL expression modulation in response to biotic stress in two contrasting commercial cultivars

Fig. 6

Expression profiles of cotton DCLs analyzed by RT-qPCR after CLRDV virus infection. Cotton plants from the cv. Fibermax that is susceptible to CLRDV infection and Delta Opal that is resistant to CLRDV infection, were inoculated with CLRDV vectors. Vectors were eliminated 24 h after inoculation. a Systemic leaves of Fibermax 966 and Delta Opal plants were collected at 24 hpi and 5, 15 and 25 dpi for the evaluation of each DCL level over time. The fold change was calculated using the 2-ΔΔCt method as described by [52]. The standard deviation is indicated by the error bars, and “*” indicates significant differences, calculated using the GraphPad Prism program. * P < 0.1 ** P < 0.01 *** P < 0.001. hpi - hours post-virus infection, dpi - days post-virus infection. b A Nested RT-PCR for CLRDV capsid protein (CP) gene amplification was performed in all systemic leaves to check virus presence in virus-free aphid inoculated (A), CLRDV infected (V) and uninoculated plants (C). The number 1 and 2 represent independent pools of treated plants. FM – CLRDV susceptible plant and DO – CLRDV resistant plant

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BMC Plant Biology

ISSN: 1471-2229

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