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Table 1 Analysis of lignin monolignols and interunit linkage in OXPdWND3A transgenic plants. Structural information of lignin was obtained by 2D 1H-13C HSQC NMR analysis. The contents of monolignols (S and G), PB (p-hydroxybenzoate), and lignin interunit linkage [β-aryl ether (β-O-4), phenylcoumaran (β-5), and resinol (β-β)] were calculated as a fraction of total lignin subunits (S + G). Two biological replicates of stem tissues were used for the analysis. The number in parenthesis displayed standard deviation

From: PdWND3A, a wood-associated NAC domain-containing protein, affects lignin biosynthesis and composition in Populus

 

WV94

Control

OXPdWND3A-1

OXPdWND3A-2

Lignin subunits

 S

56 (0.81)

61 (3.89)*

67 (1.12)*

 G

44 (0.81)

39 (2.89)*

33 (1.12)*

 S/G

1.29

1.54

2.07

 PB

7 (0.45)

10 (0.21)

2 (0.59)

Lignin interunit linkages

 β-O-4

54 (2.19)

58 (3.74)

59 (4.39)

 β-5

2 (0.78)

4 (1.39)

2 (1.45)

 β-β

2 (0.83)

4 (0.46)*

6 (0.5)*

  1. *Statistical significance against WV94 (p < 0.05)