Fig. 6

Subcellular localization and Transactivation of PabHLH1. The expression cassettes of PabHLH driven by 35S promoter, was transiently expressed in the tobacco leaves (a) and onion epidermal cells (b). The GFP fluorescence is shown in green and the DAPI fluorescence is shown in blue. c Four truncated fragments and the full-length protein were each introduced into pGBKT7, and transformed yeasts selected on both SD-Trp and SD-Trp-His-Ade. Construct containing PabHLH1 residues 1–460 and the full length protein was functional and supported survival on the multiple amino acid deficient medium. The black filled bar indicates the PabHLH1 domain