Identification and evaluation of reference genes for quantitative real-time PCR analysis in Polygonum cuspidatum based on transcriptome data

Wang, Xiaowei; Wu, Zhijun; Bao, Wenqi; Hu, Hongyan; Chen, Mo; Chai, Tuanyao; Wang, Hong

doi:10.1186/s12870-019-2108-0

Table 2 Gene expression stability under multiple conditions ranked by △CT, BestKeeper, NormFinder, geNorm and RefFinder

From: Identification and evaluation of reference genes for quantitative real-time PCR analysis in Polygonum cuspidatum based on transcriptome data

Groups	Ranking	RefFinder		△CT		BestKeeper		Normfinder		geNorm
Groups	Ranking	Gene	Stability	Gene	Stability	Gene	Stability	Gene	Stability	Genes	Stability
Abiotic stress	1	EF-1γ	1.86	NDUFA13	0.64	eIF6A	0.89	NDUFA13	0.17	60SrRNA	0.28
	2	NDUFA13	1.97	EF-1γ	0.66	60SrRNA	0.93	EF-1γ	0.22	EF-1γ	0.28
	3	60SrRNA	2.06	60SrRNA	0.70	EF-1γ	0.96	60SrRNA	0.33	NDUFA13	0.35
	4	eIF6A	4.30	ACT	0.75	YLS8	0.97	UBQ	0.44	UBQ	0.47
	5	UBQ	4.68	UBQ	0.76	NDUFA13	1.01	ACT	0.47	ACT	0.53
	6	ACT	5.32	TUB	0.77	TUB	1.05	TUB	0.52	TUB	0.58
	7	TUB	6.24	eIF6A	0.79	UBQ	1.05	eIF6A	0.54	eIF6A	0.60
	8	YLS8	7.52	UBC	0.83	ACT	1.07	UBC	0.57	YLS8	0.63
	9	UBC	9.16	TUA	0.86	TUA	1.07	TUA	0.64	GAPDH	0.66
	10	TUA	9.24	YLS8	0.89	UBC	1.09	YLS8	0.72	TUA	0.69
	11	GAPDH	10.46	GAPDH	0.89	GAPDH	1.22	GAPDH	0.72	UBC	0.71
	12	SKD1	12.00	SKD1	1.46	SKD1	1.41	SKD1	1.39	SKD1	0.83
Hormone stimuli	1	NDUFA13	1.97	NDUFA13	0.49	ACT	0.33	NDUFA13	0.26	60SrRNA	0.34
	2	SKD1	2.63	SKD1	0.50	SKD1	0.36	SKD1	0.26	EF-1γ	0.34
	3	eIF6A	3.41	eIF6A	0.50	NDUFA13	0.43	eIF6A	0.28	eIF6A	0.36
	4	EF-1γ	3.98	YLS8	0.51	UBC	0.44	YLS8	0.31	YLS8	0.39
	5	YLS8	4.43	EF-1γ	0.53	eIF6A	0.44	EF-1γ	0.33	NDUFA13	0.39
	6	60SrRNA	4.46	60SrRNA	0.53	YLS8	0.45	60SrRNA	0.34	SKD1	0.40
	7	ACT	5.62	UBQ	0.56	UBQ	0.47	UBQ	0.39	UBC	0.42
	8	UBC	6.51	UBC	0.58	TUB	0.52	UBC	0.42	UBQ	0.45
	9	UBQ	7.24	GAPDH	0.59	EF-1γ	0.52	GAPDH	0.43	GAPDH	0.46
	10	GAPDH	8.74	ACT	0.62	GAPDH	0.52	ACT	0.47	ACT	0.49
	11	TUB	10.46	TUB	0.66	60SrRNA	0.56	TUB	0.51	TUB	0.52
	12	TUA	12.00	TUA	0.92	TUA	0.79	TUA	0.84	TUA	0.58
Different tissues	1	NDUFA13	1.57	NDUFA13	0.31	GAPDH	0.92	ACT	0.06	NDUFA13	0.10
	2	EF-1γ	2.45	EF-1γ	0.32	NDUFA13	1.03	NDUFA13	0.10	EF-1γ	0.10
	3	ACT	2.63	ACT	0.32	EF-1γ	1.07	60SrRNA	0.10	60SrRNA	0.13
	4	60SrRNA	3.13	60SrRNA	0.32	60SrRNA	1.08	EF-1γ	0.13	ACT	0.16
	5	GAPDH	4.43	eIF6A	0.37	TUB	1.11	eIF6A	0.22	TUB	0.19
	6	TUB	5.69	TUB	0.38	ACT	1.19	UBQ	0.26	GAPDH	0.21
	7	eIF6A	6.32	UBQ	0.39	TUA	1.23	TUB	0.27	UBQ	0.24
	8	UBQ	7.17	GAPDH	0.42	eIF6	1.24	GAPDH	0.32	eIF6A	0.27
	9	YLS8	9.24	YLS8	0.46	UBQ	1.30	YLS8	0.38	YLS8	0.31
	10	TUA	10.49	UBC	0.54	YLS8	1.34	SKD1	0.47	UBC	0.35
	11	UBC	10.49	SKD1	0.54	UBC	1.40	UBC	0.50	SKD1	0.38
	12	SKD1	10.98	TUA	0.57	SKD1	1.46	TUA	0.54	TUA	0.41
All	1	NDUFA13	2.28	NDUFA13	0.68	YLS8	0.78	NDUFA13	0.21	60SrRNA	0.31
	2	EF-1γ	2.30	EF-1γ	0.68	eIF6A	0.84	EF-1γ	0.24	EF-1γ	0.31
	3	60SrRNA	3.08	60SrRNA	0.71	ACT	0.90	60SrRNA	0.31	NDUFA13	0.38
	4	UBQ	4.00	UBQ	0.75	UBQ	0.91	UBQ	0.42	UBQ	0.48
	5	eIF6A	4.36	ACT	0.77	UBC	0.93	ACT	0.46	eIF6A	0.54
	6	ACT	4.79	eIF6A	0.78	TUB	1.00	eIF6A	0.50	TUB	0.57
	7	YLS8	5.62	TUB	0.80	EF-1γ	1.01	TUB	0.50	ACT	0.59
	8	TUB	6.48	UBC	0.83	GAPDH	1.02	UBC	0.53	GAPDH	0.61
	9	UBC	7.33	GAPDH	0.83	60SrRNA	1.04	GAPDH	0.58	UBC	0.63
	10	GAPDH	8.49	YLS8	0.94	NDUFA13	1.04	YLS8	0.77	YLS8	0.66
	11	TUA	11.00	TUA	1.05	TUA	1.29	TUA	0.85	TUA	0.72
	12	SKD1	12.00	SKD1	1.62	SKD1	1.93	SKD1	1.54	SKD1	0.87

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ISSN: 1471-2229

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