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Table 2 Gene expression stability under multiple conditions ranked by CT, BestKeeper, NormFinder, geNorm and RefFinder

From: Identification and evaluation of reference genes for quantitative real-time PCR analysis in Polygonum cuspidatum based on transcriptome data

GroupsRankingRefFinderCTBestKeeperNormfindergeNorm
GeneStabilityGeneStabilityGeneStabilityGeneStabilityGenesStability
Abiotic stress1EF-1γ1.86NDUFA130.64eIF6A0.89NDUFA130.1760SrRNA0.28
2NDUFA131.97EF-1γ0.6660SrRNA0.93EF-1γ0.22EF-1γ0.28
360SrRNA2.0660SrRNA0.70EF-1γ0.9660SrRNA0.33NDUFA130.35
4eIF6A4.30ACT0.75YLS80.97UBQ0.44UBQ0.47
5UBQ4.68UBQ0.76NDUFA131.01ACT0.47ACT0.53
6ACT5.32TUB0.77TUB1.05TUB0.52TUB0.58
7TUB6.24eIF6A0.79UBQ1.05eIF6A0.54eIF6A0.60
8YLS87.52UBC0.83ACT1.07UBC0.57YLS80.63
9UBC9.16TUA0.86TUA1.07TUA0.64GAPDH0.66
10TUA9.24YLS80.89UBC1.09YLS80.72TUA0.69
11GAPDH10.46GAPDH0.89GAPDH1.22GAPDH0.72UBC0.71
12SKD112.00SKD11.46SKD11.41SKD11.39SKD10.83
Hormone stimuli1NDUFA131.97NDUFA130.49ACT0.33NDUFA130.2660SrRNA0.34
2SKD12.63SKD10.50SKD10.36SKD10.26EF-1γ0.34
3eIF6A3.41eIF6A0.50NDUFA130.43eIF6A0.28eIF6A0.36
4EF-1γ3.98YLS80.51UBC0.44YLS80.31YLS80.39
5YLS84.43EF-1γ0.53eIF6A0.44EF-1γ0.33NDUFA130.39
660SrRNA4.4660SrRNA0.53YLS80.4560SrRNA0.34SKD10.40
7ACT5.62UBQ0.56UBQ0.47UBQ0.39UBC0.42
8UBC6.51UBC0.58TUB0.52UBC0.42UBQ0.45
9UBQ7.24GAPDH0.59EF-1γ0.52GAPDH0.43GAPDH0.46
10GAPDH8.74ACT0.62GAPDH0.52ACT0.47ACT0.49
11TUB10.46TUB0.6660SrRNA0.56TUB0.51TUB0.52
12TUA12.00TUA0.92TUA0.79TUA0.84TUA0.58
Different tissues1NDUFA131.57NDUFA130.31GAPDH0.92ACT0.06NDUFA130.10
2EF-1γ2.45EF-1γ0.32NDUFA131.03NDUFA130.10EF-1γ0.10
3ACT2.63ACT0.32EF-1γ1.0760SrRNA0.1060SrRNA0.13
460SrRNA3.1360SrRNA0.3260SrRNA1.08EF-1γ0.13ACT0.16
5GAPDH4.43eIF6A0.37TUB1.11eIF6A0.22TUB0.19
6TUB5.69TUB0.38ACT1.19UBQ0.26GAPDH0.21
7eIF6A6.32UBQ0.39TUA1.23TUB0.27UBQ0.24
8UBQ7.17GAPDH0.42eIF61.24GAPDH0.32eIF6A0.27
9YLS89.24YLS80.46UBQ1.30YLS80.38YLS80.31
10TUA10.49UBC0.54YLS81.34SKD10.47UBC0.35
11UBC10.49SKD10.54UBC1.40UBC0.50SKD10.38
12SKD110.98TUA0.57SKD11.46TUA0.54TUA0.41
All1NDUFA132.28NDUFA130.68YLS80.78NDUFA130.2160SrRNA0.31
2EF-1γ2.30EF-1γ0.68eIF6A0.84EF-1γ0.24EF-1γ0.31
360SrRNA3.0860SrRNA0.71ACT0.9060SrRNA0.31NDUFA130.38
4UBQ4.00UBQ0.75UBQ0.91UBQ0.42UBQ0.48
5eIF6A4.36ACT0.77UBC0.93ACT0.46eIF6A0.54
6ACT4.79eIF6A0.78TUB1.00eIF6A0.50TUB0.57
7YLS85.62TUB0.80EF-1γ1.01TUB0.50ACT0.59
8TUB6.48UBC0.83GAPDH1.02UBC0.53GAPDH0.61
9UBC7.33GAPDH0.8360SrRNA1.04GAPDH0.58UBC0.63
10GAPDH8.49YLS80.94NDUFA131.04YLS80.77YLS80.66
11TUA11.00TUA1.05TUA1.29TUA0.85TUA0.72
12SKD112.00SKD11.62SKD11.93SKD11.54SKD10.87