Table 1 Details of candidate reference genes and target genes used for RT-qPCR in Polygonum cuspidatum
Gene | Gene description | Primer sequence (5′-3′) Forward/Reverse | Product (bp) | E (%) | R2 | NR accession | Arabidopsis Ortholog |
|---|---|---|---|---|---|---|---|
ACT | actin 7 | F:GCCGTCTATGATTGGAATGG R:TACCGTACAAGTCCTTCCTAA | 99 | 98 | 0.999 | MK288156 | AT5G09810 |
TUA | Tubulin-alpha 6 | F:CCAGATGCCAAGTGACAAAAC R:TTGTCTGTATGTTCCAGTCCT | 154 | 86 | 0.998 | MK288157 | AT4G14960 |
TUB | Tubulin-beta 2 | F:ATCCGACACTGTTGTTGAGC R:CCAAAGGATGGGGTTGAAAG | 144 | 86 | 0.998 | MK288158 | AT5G23860 |
GAPDH | Glyceraldehyde-3 phosphate dehydrogenase | F:CAGTGACTGTTTTCGGTTGC R:AGCCTTGTCCTTGTCGGTGA | 107 | 91 | 0.999 | MK288159 | AT1G13440 |
EF-1γ | Elongation factor 1-gamma | F:GTCATCCCTGATTGATTACGC R:GTGGGCAATAAAGCCAAGAC | 112 | 95 | 0.998 | MK288160 | AT1G57720 |
UBQ | Ubiquitin domain-containing protein | F:AGTCCTCAACTTCGTGCTATG R:TTCTGGAGGCACATTTGGAGT | 294 | 89 | 0.995 | MK288161 | AT2G17200 |
UBC | Ubiquitin-conjugating enzyme | F:ATTTGATGGCGTGGAGTTGC R:AGGGGTAAACATTGGGGTGG | 156 | 98 | 0.999 | MK288162 | AT3G57870 |
60SrRNA | 60S ribosomal RNA | F:ACTGTGATTTCGCAGACGCA R:CCTGGTGCTTGGTGAGACGG | 124 | 98 | 0.999 | MK288163 | AT5G02610 |
eIF6A | Eukaryotic translation initiation factor 6A | F:CGGATCTTGACAGGGAAACC R:AACGGCACCTGAAGGAGTGT | 187 | 94 | 0.997 | MK288164 | AT3G55620 |
SKD1 | Suppressor of K+ transport growth defect1 | F:GGCGATGGTGAGGGAGATGA R:ACCCAGCCACATCATTCCAC | 106 | 104 | 0.997 | MK288165 | AT2G27600 |
YLS8 | Thioredoxin-like protein YLS8 | F:AGATCAACTGGGCTCTAAAGG R:AATCACCAGACCTCGACCCT | 92 | 103 | 0.998 | MK288166 | AT5G08290 |
NDUFA13 | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A | F:ATGTACCAGGTCGGCGTAGG R:TCCTTCATAATTCTGGCTTCC | 161 | 98 | 0.998 | MK288167 | AT1G04630 |
Target gene | |||||||
 PcMYB4 | Transcription repressor MYB4 | F:TTGATACCGCTACAACCACA R:CACCCGTCACAACGCTATT | 196 | 96 | 0.994 | MK288154 | AT4G38620 |
 PcPAL | Phenylalanine ammonia-lyase | F:AGAACAGGATCAAGGAAT R:AACAAGGAATCAATCATC | 158 | 96 | 0.997 | MK288155 | AT2G37040 |
 PcSTS | Stilbene synthases | F:CCAGACCTAACAGTTGAGA R:TCGCACCATCAGATTCA | 80 | 90 | 0.997 | EU647245 | – |