Fig. 2

Component 1 and 2 score plots of principal component analysis of (a) Non-targeted LC-MS analysis, and (b) LC-MS targeted analysis and (c) loadings plot of LC-MS targeted analysis where significant (p < 0.05) features altered in ECU72 (pink) and COL2246 (green) are highlighted. Green and pink symbols represent infestation time-points of susceptible variety COL2246 and resistant variety ECU72, respectively. Collection times during infestation were defined by the following symbols: ⃝ 0 days post-infestation (T0); ▼ 0.5 day (12 h) post-infestation (T1); ▲ 1 day post-infestation (T2); ■ 7 days post-infestation (T3); ✦ 14 days post-infestation (T4) and ★ 22 days post-infestation (T5). Principal component analysis plots were performed using Simca software and using pareto-scaling method. Averaged biological and technical replicates are presented to facilitate visualisation