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Fig. 4 | BMC Plant Biology

Fig. 4

From: Characterization and T-DNA insertion sites identification of a multiple-branches mutant br in Betula platyphylla × Betula pendula

Fig. 4

Identification of T-DNA insertion sites in br. a The detection of BpCCR1 gene by PCR in 35S::BpCCR1 plasmid (lane 1), negative control (lane 2), and four-year-old WT (lane 3), br (lane 4), 35S::BpCCR1#OE2 (lane 5), 35S::BpCCR1#OE3 (lane 6) and 35S::BpCCR1#OE4 (lane 7) lines. The lane M is DL2000 DNA marker. b Transcript level of BpCCR1 in four-year-old BpCCR1 over-expression transgenic lines and WT by qRT-PCR. Values are mean ± standard error of three measurements. c The insertion site of br on Chr5, it shows the normal BpCOI1 structure. d The BpCOI1 structure after inserting T-DNA. The white boxes are 5’UTR, the black boxes are exons, the lines are introns, and the region separated by vertical lines are deletions. The blue box is insertion. e The insertion site of br on Chr2, it shows the normal Chr2 structure. f The Chr 2 structure after inserting T-DNA. F0, F1, F2 and F3 are the chromosome segments. F2 indicates the deletion, and blue box is insertion. g: Verification of two insertion sites by PCR. M is DL2000 DNA marker. The first three lanes are I1L, which show the amplification in WT, OE2 and br, about the LB on Chr5 based on primers I1LF and I1LR. The middle three lanes are I1R, which display the amplification about the RB on Chr5 in tested lines based on primers I1RF and I1RR. The last three lanes are I2, which indicates the amplification of RB on Chr2 in three lines based on primers I2F and I2R

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