Table 2 Tiered k (mismatches) levels applied for the search of off-targets in the wheat genome
From: WheatCRISPR: a web-based guide RNA design tool for CRISPR/Cas9-mediated genome editing in wheat
PAM | Off-target region | k (mismatches) |
|---|---|---|
Canonical (NGG) | Exons and promoters | 6 |
Introns and UTRs | 4 | |
Intergenic | 3 | |
Non-canonical (NAG, NCG, NGA) | Exons and promoters | 4 |
Introns and UTRs | 3 | |
Intergenic | 2 |