Table 1 Survey of PAM sites in the IWGSC v1.0 Chinese Spring wheat reference genome sequence
From: WheatCRISPR: a web-based guide RNA design tool for CRISPR/Cas9-mediated genome editing in wheat
Region | Canonical (NGG) | Non-canonical (NAG, NCG and NGA) |
|---|---|---|
Coding | 33,543,850 | 93,876,010 |
Promoter | 46,236,876 | 133,448,536 |
UTR/Intron | 32,345,815 | 101,719,313 |
Intergenic | 1,520,739,628 | 4,374,331,934 |