Fig. 3
From: WheatCRISPR: a web-based guide RNA design tool for CRISPR/Cas9-mediated genome editing in wheat
Outputs from the WheatCRISPR web application. Exemplary gRNA and gene plots for the phyotoene desaturase (PDS) produced using the WheatCRISPR web application are shown: a gRNAs specifically targeting TraesCS4B02G300100, and b gRNAs targeting all three homoeologues of the PDS gene (TraesCS4B02G300100, TraesCS4A02G004900, TraesCS4D02G299000). The gRNA plot displays a visualization of the rs2 and CFD scores and the score for any off-target hits to homoeologues. The blue-gray bars show the rs2 (on-target activity) score, the black points indicate the worst CFD (off-target activity) scores for each region (coding, promoter, other genic, intergenic), and the green points, if any, show the CFD scores for homoeologues. The gene plot displays the physical location of the gRNAs against the gene models. Each row in the gene model represents an isoform of the gene. The gray lines indicate introns and orange bars indicate exons. The thinner orange bars indicate UTRs