Fig. 2From: WheatCRISPR: a web-based guide RNA design tool for CRISPR/Cas9-mediated genome editing in wheatFlowchart of the steps involved in creating the gRNA database. The workflow comprises three major steps, including (1) genome-wide scanning and extraction of PAM sites and adjoining gRNA sequences, (2) mapping off-targets and (3) computing on-target activity (rs2) and cutting frequency determination (CFD) scores based on the Doench prediction models (Doench et al., 2016)Back to article page