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Fig. 5 | BMC Plant Biology

Fig. 5

From: Identification of Bna.IAA7.C05 as allelic gene for dwarf mutant generated from tissue culture in oilseed rape

Fig. 5

a. IAA7 gene structure is divided into four domains. A SNP identified between the G7 dwarf mutant and WT caused a G (WT) to E (G7 mutant) amino acid substitution within domain II of Bna.IAA7.C05. b. Gene expression of Bna.IAA7.C05 between WT (W), dwarf mutant (D) and F1 lines across multiple tissues was detected by RT-PCR. BnaActin was used as control. c. Variation within the recognition site of restriction enzyme FokI (blue boxed sequence) was used to develop a dCAPs marker for the SNP within Bna.IAA7.C05. d. Plant height of F2 individuals co-segregated with the dCAPs marker (* plant died at pod maturation)

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