Fig. 1

a Alignment of the predicted amino acid sequences of FaPRE1, FaPRE2 and FaPRE3 proteins. b-d Analysis by qRT-PCR of FaPRE genes expression in different tissues of Fragaria x ananassa “Camarosa” plants. b Analysis of FaPRE genes expression in some selected developing receptacles compared with vegetative tissues. Analysis of FaPRE genes expression in receptacles (c) and achenes (d) in different stages of development. Rec G1 and G1, small-sized green fruit; G2, middle-sized green fruits; G3, full-sized green fruit (G1 and G3: stages of development); Rec W and W, white stage; Rec R and R, ripe stage; OR, overripe stage; SN, senescent stage. Results were obtained using 3’UTR specific primers and quantification is based on Ct values. Relative expression values were calculated relative to receptacles G1 stage Ct value, which was assigned an arbitrary value equal to unity. Values are mean ± SD of five independent experiments. Statistical significance with respect to the reference sample was determined by the Student’s t-test: **p < 0.01, ***p < 0.001