Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines

Table 1 Measured ODAP contents in % w/w in drilled individual seeds of grass pea, along with literature values. Literature values were obtained by triplicate measurements of milled bulked seed samples and thus do not reflect biological variation between individual seeds

Genotype	LCMS	std. error	Rao	std. error	Literature value	std. error	Reference
LSWT11	0.416	± 0.021	0.374	± 0.041	n/a	n/a	n/a
LS007	0.261	± 0.027	0.197	± 0.016	n/a	n/a	n/a
Nirmal	0.309	± 0.017	0.285	± 0.029	0.098	± 0.003	[39]
LS8246	0.052	± 0.016	0.036	± 0.012	0.033	± 0.004	[40]
Mahateora	0.030	± 0.001	0.039	± 0.001	0.046	± 0.001	[39]
Ceora	0.029	± 0.004	0.027	± 0.004	0.065	± 0.025	[21]
P-24	0.077	± 0.002	0.070	± 0.002	0.053	± 0.001	[39]
Pea	nil	n/a	0.008	n/a	n/a	n/a	n/a
Chickpea	nil	n/a	0.003	n/a	n/a	n/a	n/a

ISSN: 1471-2229