Skip to main content
Fig. 8 | BMC Plant Biology

Fig. 8

From: Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines

Fig. 8

Chromatograms of AccQ-Tag™-derivatised plant material and standards. a: Mass transition 347 → 171 for derivatised 12C-ODAP standard. b: Mass transition 349 → 171, for derivatised di-13C-ODAP internal standard, in a sample of chickpea seed meal with a 1% admixture of LSWT11 grass pea meal; note that the internal standard peak is clearly-defined, with no nearby interfering peaks. c: Mass transition 347 → 171 in the same sample as (B); note the clearly defined peak corresponding to derivatised 12C-ODAP derived from the sample. d: Mass transition 347 → 171 in a sample of chickpea seed meal without admixture; note that the ODAP peak is now missing. Taken together, traces (C) and (D) indicate the clarity and specificity with which the LC-MS-based assay can detect ODAP at a level corresponding to 0.004% in a biological background of seed meal

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us