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Fig. 1 | BMC Plant Biology

Fig. 1

From: The long non-coding RNA lncRNA973 is involved in cotton response to salt stress

Fig. 1

Characteristics of lncRNA973. a. Schematic diagram of the location of lncRNA973 in a chromosome. Yellow boxes show the exon of lncRNA973. P1 and P2 indicated the primers used for qRT-PCR to detect the expression level of lncRNA973 X1 and lncRNA973 X2, respectively. b. Expression levels of lncRNA973 transcripts in cotton stem, root and cotyledon, determined by qPCR. c. Expression levels of lncRNA973 X1 and X2 in cotton true leaves treated with 250 mM NaCl at different time points, determined by qPCR. The lncRNA973 expression level was normalized by the GhUBQ7 expression level. The relative expression levels of lncRNA973 in stem and NaCl-0 h were assigned a value of 1, respectively. Error bars indicate ±SD of three biological replicates, with each measured in triplicate. Samples marked with different letters show a significant difference at p < 0.05. d. Analysis of the coding potential of lncRNA973. Coding potential scores were generated using the CPC program. PHO2 and GhActin are provided as coding examples, COLDAIR and Xist represent non-coding examples

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