Fig. 1

Phylogenetic comparison of AsFAD2s and other plants FAD2s. The phylogenetic tree was generated by MEGA6.0. FAD2 desaturases (FAD), hydroxylases (OH), epoxygenases (EPOX), acetylenases (ACET) and conjugases (CONJ) from various plants were included in the alignment. The GenBank accession numbers of the amino acid sequences represented in the phylogenetic tree were: AhFAD2–1, ACZ06072.1; AhFAD2–2, AHN60569.1; sixteen AsFAD2 proteins; AtFAD, AAM61113.1; BnFAD2–1, AAF78778.1; BnFAD2–2, AAS92240.1; CaACET, ABC00769.1; CoCONJ, AAK26632.1; CpEPOX, CAA76156.1; CtFAD2–1, AGC65498.1; CtFAD2–2, AGC65499.1; CtFAD2–3, AGC65500.1; CtFAD2–4, AGC65501.1; CtFAD2–5, AGC65502.1; CtFAD2–6, AGC65503.1; CtFAD2–7, AGC65504.1; CtFAD2–8, AGC65505.1; CtFAD2–9, AGC65506.1; CtFAD2–10, AGC65507.1; CtFAD2–11:ACET, AGC65508.1; DcACET, AAO38033.1; DcFAD2:OH, AAK30206.1; DsACET, AAO38036.1; FvACET, AAO38034.1; GhFAD2–1, CAA65744.1; GhFAD2–2, CAA71199.1; GmFAD2–1, AAB00859.1; GmFAD2–2, AAB00860.1; HaFAD2–1, AAL68981.1; HaFAD2–2, AAL68982.1; HaFAD2–3, AAL68983.1; HaACET, ABC59684.1; HhACET, AAO38031.1; NtFAD, AAT72296.2; OeFAD2–1, AAW63040.1; OeFAD2–2, AAW63041.1; RcOH, AAC49010.1; RhACET, AAO38035.1; SiFAD2–1, XP_011075145.1; SiFAD2–2, XP_011080227.1; and SlEPOX, AAR23815.1 (Ah, Arachis hypogaea; As, Artemisia sphaerocephala; At, Arabidopsis thaliana; Bn, Brassica napus; Ca, Crepis alpine; Co, Calendula officinalis; Cp, Crepis palaestina; Ct, Carthamus tinctorius; Dc, Daucus carota; Ds, Dimorphotheca sinuate; Fv, Foeniculum vulgare; Gh, Gossypium hirsutum; Gm, Glycine max; Ha, Helianthus annuus; Hh, Hedera helix; Nt, Nicotiana tabacum; Oe, Oleaeuropaea; Rc, Ricinus communis; Rh, Rudbeckia hirta; Si, Sesamum indicum; Sl, Stokesia laevis)