Fig. 4

The male gametophyte development of Suaeda salsa (a-j) and Arabidopsis (k-t). The pollen development observations under the DIC microscope (a-e, k-o) and fluorescence microscope with DAPI staining (f-j, p-t). For each observation, five development stages are shown: pollen mother cell stage (a, f, k and p), four haploid microspores stage (b, g, l and q), uninucleate microspore stage (c, h, m and r), binuclear pollen stage (d, i, n and s) and tricellular pollen stage (e, j,o and t) are shown respectively. SN, sperm nucleus, VN, vegetative nucleus. Bar = 20 μm