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Table 1 Expression of OsPUB41 is induced following treatment of rice leaves with various cell wall degrading enzymes

From: Overexpression of OsPUB41, a Rice E3 ubiquitin ligase induced by cell wall degrading enzymes, enhances immune responses in Rice and Arabidopsis

Experimental parametersMicroarray data bqPCR data c
CWDEsaLipase A (from Xoo)Cellulase A (from Xoo)Cellulase (from Trichoderma viride)Xylanase (from Trichoderma viride)Pectinase (from Aspergillus niger)
Time point2 h12 h12 h12 h12 h12 h
Fold change in OsPUB41 expression3.1510.42.84.3 ± 1.162.9 ± 0.111 ± 4.6
  1. aLeaves of ten-fifteen-days-old Taichung Native-1 (TN-1) rice plants were pressure infiltrated, using a needleless syringe, with any one of the bacterial or fungal CWDEs
  2. bRelative fold change of OsPUB41 in microarray analysis observed using either Lipase A (GEO-ID: GSE53940, GSE49242) or Cellulase A (GSE8216) (p < 0.05)
  3. cRelative fold change of OsPUB41 (average value from three independent experiments, ± represents standard error) when treated with CWDEs as compared to mock treatment. OsActin was used as an internal control in qPCR for rice. Student’s two-tailed t-test for independent means was performed on delta Ct values to test for significance (p < 0.05)