Overexpression of OsPUB41, a Rice E3 ubiquitin ligase induced by cell wall degrading enzymes, enhances immune responses in Rice and Arabidopsis

Table 1 Expression of OsPUB41 is induced following treatment of rice leaves with various cell wall degrading enzymes

Experimental parameters	Microarray data ^b			qPCR data ^c
CWDEs^a	Lipase A (from Xoo)		Cellulase A (from Xoo)	Cellulase (from Trichoderma viride)	Xylanase (from Trichoderma viride)	Pectinase (from Aspergillus niger)
Time point	2 h	12 h	12 h	12 h	12 h	12 h
Fold change in OsPUB41 expression	3.15	10.4	2.8	4.3 ± 1.16	2.9 ± 0.1	11 ± 4.6

^aLeaves of ten-fifteen-days-old Taichung Native-1 (TN-1) rice plants were pressure infiltrated, using a needleless syringe, with any one of the bacterial or fungal CWDEs
^bRelative fold change of OsPUB41 in microarray analysis observed using either Lipase A (GEO-ID: GSE53940, GSE49242) or Cellulase A (GSE8216) (p < 0.05)
^cRelative fold change of OsPUB41 (average value from three independent experiments, ± represents standard error) when treated with CWDEs as compared to mock treatment. OsActin was used as an internal control in qPCR for rice. Student’s two-tailed t-test for independent means was performed on delta C_t values to test for significance (p < 0.05)

ISSN: 1471-2229