Fig. 6
OsPUB41C40A is incapable of eliciting callose deposition and tolerance to R. solani infection in Arabidopsis. The graph represents average number of callose deposits per field of view (0.075 mm2) from five-six leaves with ten different fields viewed per leaf in each experiment (a). Error bars represent standard error. Student’s two-tailed t-test for independent means was performed to test for significance (p < 0.05, represented by*). Similar results were obtained in three independent experiments with three independent transgenic lines. b. Transgenic Arabidopsis seedlings (carrying OsPUB41 or OsPUB41C40A) were infected with R. solani, either in the presence or absence of the inducer (Estradiol) of transgene expression. Infected seedlings were stained with Trypan Blue and imaged using a light microscope. A scale with scores ranging from 0 to 3 was used to assess the extent of fungal infection. Score 0 = no infection/hyphae, 1 = few unconnected hyphae, 2 = sparse continuous network of hyphae and 3 = dense network of hyphae. The graph represents the frequency (as a percentage) of a particular score from ten seedlings with forty different fields viewed per seedling in each experiment. Scores of 0, 1, 2 and 3 are represented in the graph by black, dark grey, light grey and white respectively. Seedlings from three independent transgenic lines were used for each experiment. A similar trend was observed in three independent experiments. One-way ANOVA was used to test for significance, followed by Tukey-Kramer honestly significant difference test (p < 0.05, represented by*). C40A label represents OsPUB41C40A