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Fig. 2 | BMC Plant Biology

Fig. 2

From: Overexpression of OsPUB41, a Rice E3 ubiquitin ligase induced by cell wall degrading enzymes, enhances immune responses in Rice and Arabidopsis

Fig. 2

Transient overexpression of OsPUB41 leads to enhanced expression of rice defense genes. Transcript levels of JA (a) and SA (b) biosynthetic and response genes and of PR genes (c), were measured by qPCR upon overexpression of OsPUB41 in rice. AOS2: Allene oxide synthase2, AOC: Allene oxide cyclase, LOX2: Lipoxygenase2 and OPR2 and OPR4: 12-oxophytodienoate reductase2 and 4 (JA biosynthetic genes), JAZ8 and JAZ13: Jasmonate ZIM-Domain8 and 13 (JA response genes), PAL1 and PAL2: Phenylalanine ammonia lyase1 and 2 (SA biosynthetic genes), SGT1: SA glucosyltransferase1, NH1: Non-expresser of PR1 homolog1 (SA response genes), WRKY13: SA and JA response gene. OsActin was used as an internal control in qPCR. The graph represents average fold change values from three biological replicates. Student’s two-tailed t-test for independent means was performed on delta Ct values to test for significance (p < 0.05)

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