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Fig. 7 | BMC Plant Biology

Fig. 7

From: A mutant allele of ζ-carotene isomerase (Z-ISO) is associated with the yellow pigmentation of the “Pinalate” sweet orange mutant and reveals new insights into its role in fruit carotenogenesis

Fig. 7

a Functional analysis of Z-ISO from WT, P5 and P6 cDNAs in E. coli grown under dark and light conditions. E. coli with pZETA alone was used as control. Carotenoid composition in E. coli cells was determined by HPLC-PAD as described. b Representative chromatograms for Z-ISO WT, Mutant (P5 and P6) sequences and control (pZETA) under dark and light conditions. c Spectra of 9,15,9′-tri-cis-ζ-carotene and 9, 9′-di-cis-ζ-carotene

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