Fig. 6From: A mutant allele of ζ-carotene isomerase (Z-ISO) is associated with the yellow pigmentation of the “Pinalate” sweet orange mutant and reveals new insights into its role in fruit carotenogenesisDetail of genomic sequence of intron1 (blue letter) and exon2 (black) Z-ISO isolated from ‘Navelate’ and displaying the T insertion in exon2 identified in ‘Pinalate’ mutant (a). The underlined sequence in exon2 is deleted as a result of abnormal splicing in a ‘Pinalate’ transcript variant. (b) Structures of Z-ISO transcripts isolated from ‘Navelate’ and ‘Pinalate’. Z-ISO WT transcript encodes a 374 amino acid protein and was isolated from ‘Navelate’ and ‘Pinalate’ fruits; Z-ISO P5 and P6 sequences were isolated from ‘Pinalate’. The Z-ISO P6 contains the T insertion in exon 2 (position 1587_1588) and Z-ISO P5 transcript has a 42 bp deletion at the junction of intron1-exon2 plus T insertion in exon 2 (position 1587_1588). Alterations in P5 and P6 transcripts sequences resulted in premature stop codons and generated truncated proteins. Tp, Predicted plastid transit peptideBack to article page