Fig. 2
NanoLC-MS/MS identification for phosphorylation sites of total proteins from rice leaves. a Overview of the experimental design for the phosphorylation patterns of total proteins. The total proteins were digested with trypsin. Phosphopeptides are enriched from the pooled peptide mixture with titanium dioxide (TiO2) beads and subsequently analyzed with nanoLC−MS/MS. b The distribution of peptides having one, two, three, and four and more phosphorylation sites. c The distribution of phosphorylated residues. pS, phosphoserine; pT, phosphothreonine; pY, phosphotyrosine