Fig. 4

PDX1.2 is dispensable for Arabidopsis development under standard conditions. a Photographs of PDX1 lines as indicated grown under an 8 h photoperiod (120 μmol photons m^− 2 s^− 1) at 22 °C and 16 h of darkness at 18 °C for 27 days after germination, compared to wild-type. b and (c) Genotyping of reciprocal test crosses of either CRISPR1 (pdx1.2–3) (b) or CRISPRB (pdx1.2–5) (c) with SAIL-640-D11 (pdx1.2–1) for the T-DNA amplicon in pdx1.2–1 and a PDX1.2 amplicon in the T₁ generation. Representative analyses of eight plants from each cross are shown compared to wild type (Col-0). Both amplicons can be found in approximately 50% of the plants. DNA sizing ladder is shown in the lane marked M