Fig. 1

Characterization of PDX1.2 expression in T-DNA insertion lines. a Schematic representation of PDX1.2. On the right, the single exon is depicted as a black box and the immediate upstream region is in gray and is further detailed on the left. The red box represents the heat shock element (HSE), which is from − 165 to − 153 bp upstream of the ATG translational start codon (+ 1). Alternative transcriptional start sites (TSS1 and TSS2) are indicated. The gray (TSS1) and black (protein coding sequence CDS)) arrow lines indicate the annealing positions of the primers used for qPCR. The locations of the T-DNA insertion in CS872273 (pdx1.2–2) and SAIL-640-D11 (pdx1.2–1) are as depicted and were confirmed by genotyping and sequencing. b and (c) Quantitative analysis of PDX1.2 expression relative to GAPDH in pdx1.2–2 compared to wild type (Col-0), in the absence (−HS) and presence of heat stress (+HS) using primers specific to the CDS or TSS1. Heat stress was induced by incubating seedlings for 1 h at 37 °C. d and (e) As for (b) and (c) but in hsfb1 hsfb2b compared to wild type (Col-0). Plants were grown in sterile culture under a 16-h photoperiod (120 μmol photons m^− 2 s^− 1) at 22 °C and 8 h of darkness at 18 °C. The data are the average of three biological and three technical replicates. Statistical differences from the wild type were calculated by a two-tailed Student’s t test and indicated by an asterisk for P < 0.001. In all cases, error bars represent SE