Table 1 The Twi1 in vitro glycosyltransferase activity assay. The Twi1 purified recombinant protein was tested for glycosyltransferase activity in vitro using UDP-glucose and the following phenolics as sugar acceptors: 2,4-Dihydroxybenzoic acid (2,4-DHBA), 2,4,6-Trihydroxybenzoic acid (2,4,6-THBA), Benzoic acid, Salicylic acid, 4-Hydroxybenzoic acid, Scopoletin, Esculetin, Umbelliferone, Cinnamic acid, p-coumaric acid, Caffeic acid, Ferulic acid, o-coumaric acid and Chlorogenic acid. Samples were analysed by HPLC-fluorescence or UPLC-Q-ToF-MS to detect sugar acceptor and/or glycoconjugate formation

From: Tomato glycosyltransferase Twi1 plays a role in flavonoid glycosylation and defence against virus