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Fig. 4 | BMC Plant Biology

Fig. 4

From: Tomato glycosyltransferase Twi1 plays a role in flavonoid glycosylation and defence against virus

Fig. 4

Twi1 in vitro activity assay with scopoletin as the sugar acceptor. a The chromatographic peak obtained when scopoletin was incubated with UDP-glucose in the presence of the Control sample (protein extract from the N. benthamiana leaves agroinoculated with the pGWB8 empty vector). b The chromatogram obtained upon incubation with the Twi1 purified recombinant protein expressed in the N. benthamiana agroinoculated leaves. A differential peak was detected, corresponding to scopoletin conjugated with glucose. c The product of the reaction shown in (b) was incubated with beta-glucosidase. A free scopoletin peak was the only one detected after treatment. Samples were analysed by HPLC-fluorescence

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