Genetic diversity and population structure of the sweet leaf herb, Stevia rebaudiana B., cultivated and landraces germplasm assessed by EST-SSRs genotyping and steviol glycosides phenotyping

Table 3 Polymorphism analysis at 18 loci of Stevia rebaudiana genome for 145 genotypes

Markers Name	Allele Number	Product size expected	Allele size range	Major Allele Frequency	PIC^a	Gene Diversity (He)	Heterozygosity (Ho)^b	Fis W&C^c
stvia004	17	185	166–226	0.32	0.81	0.83	0.79 ns	0.047
stvia018	13	121	89–134	0.22	0.82	0.84	0.46***	0.452
stvia021	5	119	97–121	0.38	0.60	0.67	0.47***	0.299
stvia024	16	205	173–220	0.25	0.83	0.84	0.69***	0.18
stvia025	15	224	203–243	0.21	0.84	0.86	0.70***	0.193
stvia036	15	163	144–180	0.28	0.83	0.85	0.87***	−0.021
stvia044	9	208	203–221	0.36	0.70	0.74	0.49***	0.341
stvia048	19	153	132–163	0.31	0.82	0.84	0.84 ns	0.002
stvia051	13	194	182–224	0.23	0.84	0.86	0.81***	0.062
stvia057	16	236	190–265	0.28	0.77	0.80	0.68***	0.156
stvia071	8	187	168–191	0.36	0.74	0.77	0.74 ns	0.038
stvia072	10	239	231–251	0.56	0.63	0.65	0.61***	0.057
stvia079	13	173	153–198	0.29	0.80	0.82	0.79***	0.035
stvia084	11	159	142–178	0.49	0.70	0.72	0.70 ns	0.041
stvia093	12	221	205–247	0.38	0.78	0.80	0.79***	0.017
stvia096	8	204	174–213	0.29	0.75	0.78	0.76***	0.033
stvia099	6	111	88–121	0.34	0.66	0.71	0.66 ns	0.076
stvia107	7	125	125–143	0.52	0.61	0.65	0.61 ns	0.062

^aPIC Polymorphic information content
^bChi-square test for Hardy-Weinberg equilibrium; ns = not significant; ***P < 0.001
^cFIS W&C FIS Weir and Cockerham (1984) [47]

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