Oligo-painting and GISH reveal meiotic chromosome biases and increased meiotic stability in synthetic allotetraploid Cucumis ×hytivus with dysploid parental karyotypes

Table 1 Meiotic chromosome behavior of five chromosomes at metaphase I and anaphase I in F₁, S₄ and S₁₄ generations

Plant group	No. of PMCs at MI		No. (%) of PMCs with homologous bivalents		No. of PMCs with univalents		No. of PMCs with homoeologous pairings
Plant group	C5-oligo	C7-oligo	C5/H9/H10	C7/H1	C5/H9/H10	C7/H1	C5 and H9/H10	C7 and H1
F1	132	106	–	–	86/92/103	87/87	29/17	19
S4	123	112	75 (61)	101 (90.2)	16/9/38	6/7	0/11	0
S14	137	104	119 (86.9)^**	100 (96.2)^*	5^*/3^/14^***	2/2^*	0/3	0

MI metaphase I, AI anaphase I, PMC pollen mother cell; statistical test for comparisons with S4 (t-test): ^*, p < 0.05; ^**, p < 0.01; ^*** p < 0.001

ISSN: 1471-2229