Fig. 8
Meiotic behavior at metaphase I in S14 and the biases of chromosomes/subgenomes for univalents and lagged bivalents at metaphase I. a Two homologous bivalents of C7 and H1 at metaphase I in S14 generation. b Three homologous bivalents of C5, H9 and H10 at metaphase I in S14 generation. c GISH image showing normal meiotic behavior of two subgenomes at metaphase I in S14 generation. d and e Only one H-bivalent lagged behind the other bivalents and did not reach the equatorial plate at metaphase I (white arrows). Bars = 5 μm. Five chromosomes (H1, C7, C5, H9 and H10) and subgenome biases for the occurrence of univalents and lagging bivalents at MI of S4 and S14 were quantified by oligo-painting and GISH and are presented in (f) and (g) respectively. The x-axes in (f) and (g) refer to the five chromosomes and the different generations (S4 and S14) respectively, while the y-axes in (f) and (g) refer to the frequency of univalent(s) and lagged bivalent(s). Error bars indicate the ±SD over three biological replicates. Asterisks indicate statistically significant differences at *, p < 0.05; **, p < 0.01; *** p < 0.001