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Table 3 qRT-PCR primers for transcript profiling in Ae. tauschii accessions

From: Phenotypic and molecular characterization of Hessian fly resistance in diploid wheat, Aegilops tauschii

Gene

Forward Primer

Reverse Primer

Ubqa (Ubiquitin)

ggtgtctccggtatcctccaa

tgctccacaccagcagaagt

Hessian fly-responsive biomarker:

 Hfr-1 (Hessian fly-response gene 1)

cttaagacctctgctttctctaggtga

gatggtgatgcgctctaaacg

 Hfr-3 (Hessian fly-response gene 3)

gtccttgctgggctgatctc

tccggtcctaggccacagta

 Mds-1 (Mayetiola destructor susceptibility 1)

ccaaaagcagacagcaaccccaacc

gtcggcgaaggggtcgaacac

 Cer4 (Fatty acyl CoA reductase)

ccgattccgcattcaacttt

gacaccagggatgtggacctt

Oxidative stress pathway:

 Prx (Class III peroxidase)

agggcgccttcttcgag

aggtccatgttgctcatcttgg

 Nox (NADPH-dependent oxidase)

atgttcggcaacttggtgact

cgtctgctctaagaagaccactttt

 Gst (Glutathione S-transferase)

gtgccggtgctgatcca

ggcgaaagcctcgtcgat

Secondary metabolite biosynthesis:

 Pal (Phenylalanine-ammonia lyase)

gcgtgaagacagtggctagga

gcgtgcgttgtggagatg

 4Cl (4-coumarate-CoA ligase)

gcgaagcaggtggtgttctac

gggatggagctcacgaagaag

 Ccr (Cinnamoyl-CoA reductase)

gttgggccctctgctacaga

caccgagccgtccagatact

 HfrDrd (Hessian fly-responsive disease resistance dirigent-like gene)

ttgaccagtcccaccgaca

attcaaagtgttccgtaggacg

  1. aGene used as endogenous control