Fig. 6

The flavonoid pathway modulated in response to ERI treatment. RT-qPCR analysis of the flavonoid pathway genes (a) F3’5’H, (b) F3’H, (c) CHS, (d) CHI, (e) F3H, and (f) DFR in 1-day ovules and in 5 and 10-day-cultured fibres for the control and ERI treatments. Quantification of different flavonoids (NAR, DHK, K, DHQ, Q and ERI) in Control and ERI samples after (g) 1 day, (h) 5 days and (i) 10 days of culture. For a-i, error bars represent the SD based on three technical repeats. Significant differences were determined and are denoted as * at 5% and ** at 1% significance, while non-significant differences are indicated by ns. (j) FPKM values of flavonoid pathway genes derived from RNA-seq analysis are plotted in a heat map as log2 values of the ERI/control ratios