Fig. 2

Molecular structure and phylogenic tree of new subgroup members of AP2/EREBPs. TcA3Bz1 and its homologues belong to a new subgroup AP2/EREBPs that contained three AP2-domains. Their molecular structures were highly different. a Besides of AP2 domain, each homologues of TcA3Bz1 had different conserved domains, including B3_DNA-binding domain, zf-C3Hc3H-domain, Neuromodullin_N superfamily domain and Agenet domain. b Alignment of the three AP2 domains of the new AP2/EREBP subgroup proteins. Red stars indicated the conserved residues of AP2 domain. c Phylogenetic analysis of the three AP2 domains. Each repeat of the three AP2 domains of TcA3Bz1, Gb_05581, Pp3c11_13860V3.1.p, Pp3c7_17700V3.6.p and PTQ32953.1 was clustered separately, indicating R1, R2 and R3 separately evolved. d Alignment of B3-domains of new clade proteins with several Arabidopsis thaliana B3 superfamily proteins. All Arabidopsis thaliana B3 superfamily proteins were obtained from TAIR (https://www.arabidopsis.org/browse/genefamily/B3binding.jsp). e Phylogenic analysis of B3-domains. f Alignment of zf-C3Hc3h-domains of new clade proteins with a MA_10436317g0020 of Picea abies. MA_10436317g0020 were highly homologues with N-terminus of TcA3Bz1, without exception that MA_10436317g0020 only contain one AP2-domain