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Fig. 6 | BMC Plant Biology

Fig. 6

From: Elucidating the role of shikimate dehydrogenase in controlling the production of anthocyanins and hydrolysable tannins in the outer peels of pomegranate

Fig. 6

(a) Comparison of key amino acids in the SDH domain of DQD/SDHs from Arabidopsis (Arath) and pomegranate. Protein alignment was performed by the T-Coffee test. Numbering of amino acids is based on the DQD/SDH from Arabidopsis thaliana (AtSDH; [31]). Green color represents the active site residues of Arabidopsis protein. Identical amino acids at the respective positions in the pomegranate sequences are also highlighted in green; when they differ, they are highlighted in light-blue. (b) Phylogenetic tree constructed from PgSDHs and 12 plant SDH sequences available in public databases. PgSDHs are highlighted in blue. The clusters shown in the green letters are according to the classification proposed by Bontpart et al. [22]. A phylogenetic analysis was performed using the neighbor-joining method using phylogeny.fr, 0.1 substitutions per site. The sequences used for this analysis were of V. vinifera (Vv [22]; accession numbers ANC67814, ANC76816, ANC67817); Juglans regia (Jr, [16]; accession number AAW65140); Nicotiana tabacum (Nt, [32]; accession number AAS90325); Diospyros kaki (Dk, [33]; accession number BAI40147); Fragaria vesca (Fv, [34]; accession numbers XP_004302480, XP_004302479, XP_004288087); Eucalyptus sp. (Eg, [35]; accession number Eucgr.H04427.1); Solanum lycopersicum (Sl) accession number AAC17991; and Arabidopsis thaliana accession number AAF08579. Accession numbers correspond either to the NCBI or Phytozome 12 database

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