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Fig. 4 | BMC Plant Biology

Fig. 4

From: A modular steroid-inducible gene expression system for use in rice

Fig. 4

DEX-induced growth defects in transgenic lines can be ameliorated by addition of IPTG. a-d) pZmUbi:GUS and wild-type controls. e-g) T1 seedlings from 7 independent transgenic lines (at least two lines each for the inducible constructs 17203, 17610 and 17613). All seeds were germinated on ½ MS plates for 3 days and then transferred to plates containing either: 10 mM IPTG (a, e): 10 μM DEX (b, f): 10 μM DEX plus 10 mM IPTG (c, g); or ½ MS (d) and grown for 5 days. Some lines segregated non-transgenic siblings which were not affected by DEX-mediated LhGR activation (marked by red arrows and confirmed by GUS staining in Fig. 6). Scale bar = 1 cm

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