Fig. 2

GUS activity is induced by DEX in 14 independent T1 pOp6/rcoLhGR lines. GUS enzymatic activity measured in total leaf protein extracts from individuals segregating in T1 lines transformed with construct 17203 (a, e), 17610 (B, F) or 17613 (c, g). Individuals from a pZmUbi:GUS line were used as positive controls, and wild-type Kitaake rice plants (WT) were used as negative control (d). The assay used 4-methylumbelliferyl β-D-glucuronide (4-MUG) as a substrate to detect activity in the absence (grey bars) or presence (black bars) of 10 μM DEX. Bars represent the mean value from 3 technical replicates per individual ±SD. *P < 0.05 in a paired t-test. Note the maximum value on the y-axis is 3000 in (a-c), 1000 in (d) 75 in (e), and the axis is discontinuous in (f, g). Raw data are presented in Additional file 7. Nomenclature used: number indicates independent transgenic event and letters indicate segregating T1 individuals derived from each event. As such, at least four independent events were tested for each construct: lines 5, 6, 7 and 10 for 17203, lines 2, 5,7 and 8 for 17610 and lines 1, 2,6, 9, 10 and 11 for 17613. † indicates lines segregating for two linked T-DNA insertions, ‡ indicates lines segregating for three T-DNA insertions and the remaining lines have a single insertion